Management Information System (MIS) is critical for the success and performance of organizations. However, few of the previous studies examined the mechanism of MIS in improving the business performance. This study aims to explore the role of MIS effectiveness in improve the business processes and the operational excellence of the Jordanian banks. The data of this research is collected from 159 managers in two Jordanian banks: Islamic Jordanian bank and Housing bank. In order to address the research aims, the SPSS and AMOS tools are utilized to conduct several analyses such as demographic analysis, confirmatory factors analysis, and descriptive analysis. The results show that the effectiveness of MIS functions would be designed based on the business processes such as information workflow and working functionality. The MIS effectiveness includes many dimensions such as usability, conversion rare and financial benefits. The effective MIS functions plays important role in improve the operational excellence of the banks such as working innovations and services quality. This research provides useful information about the relationship between the MIS functions, business processes, and operational excellence in the banks.